ABSTRACT
Candidiasis as a common complication in patients who wear dentures. Zirconia particles and nanoparticles have antimicrobial properties. This study aimed to investigate the effect of adding zirconia particles and nanoparticles, to denture adhesive on candidiasis.METHODSA total of 46 wells in 6 columns and 6 rows were selected from a cell culture plate. Equal amounts of Candida albicans and denture adhesive were added to all wells. Zirconia was then added to the first three rows at different concentrations of particles and the second three rows received different concentrations of nanoparticles, so that the concentrations were half the previous column. After 24 hours, by adding one type of Thiazolyl Blue Tetrazolium Bromide (MTT) to all the wells, the growth or lack of growth of C. albicans was investigated. Finally, the death or survival (merely lack of growth) of C. albicans was studied by sampling the wells with no growth.RESULTSC. albicans growth was seen in all concentrations of zirconia particles. However, it did not grow in all three rows of nanoparticles up to 31 µgr/mL concentration. Further, all wells with no growth continued to grow after removal of nanoparticles from the medium. Zirconium particles may have anti-candida properties at concentrations above 250 µgr/mL. However, nanoparticles with a minimum concentration of 31 µgr/mL inhibit the growth of C. albicans but do not cause death of these microorganisms.